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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 4687154, 8 pages
Research Article

In Vitro Control of Uropathogenic Microorganisms with the Ethanolic Extract from the Leaves of Cochlospermum regium (Schrank) Pilger

1Faculty of Exact Sciences and Technology, Federal University of Grande Dourados, Dourados, MS, Brazil
2Faculty of Medicine, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil
3Faculty of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil
4Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, PR, Brazil
5Course of Chemistry, State University of Mato Grosso do Sul, Dourados, MS, Brazil
6Faculty of Biological and Environmental Science, Federal University of Grande Dourados, Dourados, MS, Brazil

Correspondence should be addressed to Kelly Mari Pires de Oliveira; rb.ude.dgfu@arieviloyllek

Received 3 August 2017; Revised 3 November 2017; Accepted 9 November 2017; Published 11 December 2017

Academic Editor: Letizia Angiolella

Copyright © 2017 Danny Ellen Meireles Leme et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The roots of Cochlospermum regium, popularly known as “algodãozinho-do-cerrado,” are used for the treatment of genitourinary infections. However, the removal of their subterranean structures results in the death of the plant, and the use of the leaves becomes a viable alternative. Therefore, the antimicrobial activity of Cochlospermum regium leaf’s ethanolic extract and its action on the biofilm formation of microorganisms associated with urinary infection were evaluated. The total phenolic compounds, flavoids, and tannins were quantified using the reagents Folin-Ciocalteu, aluminum chloride, and vanillin, respectively. The antimicrobial activity was evaluated by the broth microdilution method and the effect of the extract in the biofilm treatment was measured by the drop plate method. Cytotoxicity was evaluated by the method based on the reduction of MTS and the mutagenicity by the Ames test. The ethanolic extract of C. regium leaves presented 87.4 mg/EQ of flavonoids, 167.2 mg/EAG of total phenolic compounds, and 21.7 mg/ECA of condensed tannins. It presented reduction of the biofilm formation for E. coli and C. tropicalis and antimicrobial action of 1 mg/mL and 0.5 mg/mL, respectively. The extract showed no cytotoxicity and mutagenicity at the concentrations tested. This study demonstrated that C. regium leaves are a viable option for the treatment of genitourinary infections and for the species preservation.