trans-Cinnamaldehyde Inhibits Microglial Activation and Improves Neuronal Survival against Neuroinflammation in BV2 Microglial Cells with Lipopolysaccharide Stimulation

Figure 3

Effects of TCA on iNOS, COX-2, IL-1β, and TNF-α mRNA in LPS-stimulated BV2 cells. BV2 cells were stimulated with LPS (0.1 μg/ml) with or without TCA (10 μM) pretreatment for varying times. Total RNA was isolated and subjected to quantitate the levels of iNOS (a), COX-2 (b), IL-1β (c), and TNF-α (d) mRNA by qRT-PCR analysis. Values are expressed as fold change over the respective Controls. Each value indicates the mean ± SEM from three independent experiments. , , and versus untreated Controls; , , and versus LPS alone.

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