Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-β/c-Fos-MMP-7 Pathways

Figure 4

ANTCIN-H prevents invasion and regulates MMPs gene expression. (a) 768-0 cells were pretreated without or with 20, 50, 100, and 200 M antcin-H for 24 h and then seeded into Matrigel-coated Transwell apparatus for another 24 h in the absence or presence of antcin-H. After incubation, the invaded cells were stained with Giemsa solution and counted using a microscope. Scale bar, 100 m. The data were represented as the mean ± SD of nine replicates from three separated experiments. versus control. (b) Real-time PCR analysis of MMPs and TIMPs gene expression. Cells were treated without or with 20, 50, and 100 M antcin-H for 24 h. After treatment, the RNA extracted from 786-0 cells was subjected to a real-time PCR. -Actin was used as an internal control. Data were represented as the mean ± SD of three independent experiments. Statistically significant, , , and .

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