Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-β/c-Fos-MMP-7 Pathways

Figure 5

Suppression of Src, FAK, and ERK1/2 signaling pathways by antcin-H. (a) Time course-dependent experiment. 786-0 cells were treated without or with 100 M antcin-H for 4, 8, and 24 h. (b) Dose-dependent experiment. Cells were treated without or with 20, 50, and 100 M antcin-H for 24 h. After incubation, total protein lysates were isolated; the Western blotting analysis was performed to examine the levels of phosphorylated-Src, phosphorylated-FAK, phosphorylated-ERK1/2, phosphorylated-C/EBP-, and c-Fos. -Actin was used as an internal loading control.

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