Research Article

Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation

Figure 4

The effects of the GLGZD aqueous extract on axonal morphology and length after OGD in neonatal rat cortical slices. Immunohistochemical methods were used to label NF68 protein in brain slices, and the morphology and axon length were observed. Five fields of the cortical motor area in each slice were selected with a high-power lens. The lengths of ten random positive axons in each field were measured and averaged using ImageJ software. The mean lengths were calculated from the measured lengths of the axons in each slice. All data are expressed as the mean ± SE. versus the control group; , versus the OGD group.