Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca²⁺ Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis

Table 1

Probe, primer, and product (bp) in RT-PCR.

Gene; target	Probe	Upstream primer	Downstream primer	Product (bp)
PLC-β1	CCTCCTCATGAACTCTGGCTTC	CCAGACAGTGGATCTAGCTATG	CTGACCTGAAATAATCTTAACAG	181
PKC-α	CCTCCTCATGAACTCTGGCTTC	GCTGAGGCAGAAGAACGTGCAT	CAAAACAGCAAACTTGGCACTG	143
PKC-β1	GTCTTGGTTGTCACCCCATCCC	GAAACTTGACAACGTGATGCTG	CAACATTTCATACAGCAGGACT	205
PKC-ε	GAATCCCTTCCTTGCACATCCC	CTACAGGGATTTGAAACTGGAC	GAGCTATGTAGTCAGGAGTCCC	140
AC	TGCTATCCATCCGACTGGCCAC	CAGGCCCCAGTACGACATCTGG	GTGGACTTCCTCAGTCACCTGA	100
β-actin	TCACTGTCCACCTTCCAGCAGA	GAAGATCAAGATCATTGCTCCT	TACTCCTGCTTGCTGATCCACA	111