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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 5906539, 12 pages
https://doi.org/10.1155/2017/5906539
Research Article

Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes

1Department of Pharmacology, Doctor of Philosophy Program in Pharmacology, Faculty of Medicine and Graduate School, Chiang Mai University, Muang 50200, Thailand
2Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Muang, Chiang Mai 50200, Thailand
3Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Muang, Chiang Mai 50200, Thailand
4Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Rajathevi, Bangkok 10400, Thailand

Correspondence should be addressed to Seewaboon Sireeratawong; moc.liamg@noobawees

Received 28 April 2017; Accepted 17 July 2017; Published 16 August 2017

Academic Editor: Gorkem Kismali

Copyright © 2017 Mingkwan Na Takuathung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Psoriasis is a chronic inflammatory and immune-mediated skin disease. The pathogenesis involves T cells activation via the IL-23/Th17 axis. Conventional treatments of psoriasis have adverse events influencing patients’ adherence. Wannachawee Recipe (WCR) has been effectively used as Thai folk remedy for psoriasis patients; however, preclinical evidence defining how WCR works is still lacking. This study defined mechanisms for its antiproliferation and anti-inflammatory effects in HaCaT cells. The cytotoxicity and antiproliferation results from SRB and CCK-8 assays showed that WCR inhibited the growth and viability of HaCaT cells in a concentration-dependent manner. The distribution of cell cycle phases determined by flow cytometry showed that WCR did not interrupt cell cycle progression. Interestingly, RT-qPCR revealed that WCR significantly decreased the mRNA expression of IL-1β, IL-6, IL-8, IL-17A, IL-22, IL-23, and TNF-α but induced IL-10 expression in TNF-α- and IFN-γ-induced HaCaT cells. At the protein level determined by ELISA, WCR significantly reduced the secretion of IL-17A, IL-22, and IL-23. The WCR at low concentrations was proved to possess anti-inflammatory effect without cytotoxicity and it did not interfere with cell cycle of keratinocytes. This is the first study to provide convincing evidence that WCR is a potential candidate for development of effective psoriasis therapies.