Research Article

Effects of Wannachawee Recipe with Antipsoriatic Activity on Suppressing Inflammatory Cytokine Production in HaCaT Human Keratinocytes

Figure 4

Effects of WCR on cytokine secretion in TNF-α- and IFN-γ-stimulated HaCaT cells. ELISA was performed to determine the concentration of IL-17A, IL-22, and IL-23 in the culture supernatants of cells treated with TNF-α and IFN-γ (each 10 ng/mL) for 24 h with or without the presence of WCR (50 and 100 μg/mL). Acitretin (20 μM) was used as a positive control; n.s., not significant. Data are expressed as mean ± SEM of three independent experiments. , , and compared with TNF-α and IFN-γ treated cells.