Research Article
Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
Figure 3
Ex vivo NK cell activity. NK cells were isolated from spleens, and IFN-γ secretions and cytotoxic activities were assayed; B16 cell injections, B16 cell injections with JTT treatment, and control mice. (a) IFN-γ production in NK cells; isolated NK cells were resuspended at a density of 2 × 105 cells/well in DMEM-FCS-PSN and were cultured in 24-well plates in triplicate. B16 cells were then added to NK cells at a 1 : 20 ratio (B16 : NK). After coculture for 24 h, culture supernatants were collected, and IFN-γ levels were measured using enzyme-linked immunosorbent assay (ELISA). (b) Assays of NK cell cytotoxicity toward YCA-1 cells; YAC-1 cells at 1 × 105/well were incubated with fresh spleen cells at fixed E : T ratios for 4 h and NK cell cytotoxicity was measured using a modified WST-8 assay; ; ; ; data are expressed as means ± SD.
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