Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model

Figure 4

Influence of anti-NK antibody injections on B16 melanoma cell metastasis in mice treated with JTT. C57BL/6 mice were orally given 3.0% JTT for three weeks from one week before injections of 2 × 10⁵ B16 melanoma cells. Anti-asialo-GM1 antibody was injected intraperitoneally and numbers of tumor cell colonies on lungs were counted three weeks later. (a–e) Images of tumor cell colonies on excised lungs; (f) quantification of numbers of colonies on lungs; IgG, normal rabbit IgG; asialo, anti-asialo-GM1 mouse antibody; ; ; data are presented as means ± SD.

(a)

(b)