Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model

Figure 5

Influence of combined JTT and anti-PD-1 antibody treatments on B16 melanoma cell metastasis in mice. C57BL/6 mice were orally administered with 3.0% JTT for four weeks from the week before 2 × 10⁵ B16 melanoma cells were injected. Anti-PD-1 antibody was injected intraperitoneally and numbers of tumor cell colonies were counted four weeks later. (a–f) Images of tumor cell colonies on lungs; (g) quantification of numbers of colonies on the lungs; IgG, isotype control rat IgG; PD1, anti-PD-1 antibody; ; ; data are presented as means ± SD.

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