Iridoid Glycosides Fraction Isolated from Veronica ciliata Fisch. Protects against Acetaminophen-Induced Liver Injury in Mice

Figure 7

The effects of iridoid glycosides fraction (IGF) pretreatment on TNF-α and NF-κBp65 expression Bp65 expression in APAP-induced ALI. Representative immunohistochemical staining of TNF-α (a) and NF-κBp65 (b) in the liver was divided into negative/mild, low, moderate, and strong grades. Expression in normal control mice (c) was negative/mild. Each low magnification (200x) image was paired with a high magnification (400x) image for clearer observation. The immunohistochemical staining scores of TNF-α and NF-κBp65 were significantly lowered by IGF, which indicated a reduction in their activation and expression (d). Data are pressed as the mean ± SD (n = 8). Notes: , , compared with group II. Group I, normal control (saline); group II, model control (APAP only); group III, APAP + 150 mg/kg BDP; group IV, APAP + 450 mg/kg IGF; group V, APAP + 300 mg/kg IGF; group VI, APAP + 150 mg/kg IGF.

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