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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 6235027, 8 pages
Research Article

Pharmacodynamics and Medicinal Chemistry of an External Chinese Herbal Formula for Mammary Precancerous Lesions

1School of Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, China
2The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China
3Institute of Biomedicine, Department of Cellular Biology, Guangdong Province Key Lab of Bioengineering Medicine, Jinan University, Guangzhou, Guangdong 510632, China
4College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, China

Correspondence should be addressed to Min Ma; nc.ude.unj@nimamt

Received 24 March 2017; Accepted 24 May 2017; Published 24 July 2017

Academic Editor: Ken Yasukawa

Copyright © 2017 Ruixue Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ruyan Neixiao Cream (RYNXC) is a traditional Chinese herbal formula for treating mammary precancerous disease. This study was carried out to investigate in vivo anticancer effect of RYNXC and multiple constituents. 32 virginal Sprague-Dawley rats were randomly divided into blank control group (BC), mammary precancer models group (MODEL), tamoxifen group (TAM), and Ruyan Neixiao Cream group (RYNXC). TAM was intervened by tamoxifen; RYNXC was intervened by Ruyan Neixiao Cream. The chromatographic separation was performed by high performance liquid chromatography (HPLC) coupled with mass spectrometry (MS). RYNXC showed significant improvement in erythrocyte aggregation index (EAI), hematocrit (HCT), fibrinogen (FIB), spleen coefficient, and uterus coefficient compared with MODEL. In RYNXC and TAM groups, atypical hyperplasia was observed in pathological mammary tissues; meanwhile in MODEL group, ductal carcinoma was observed in situ. Moreover, fifteen compounds were characterized according to HPLC-MS data, including organic acids, tannin, alkaloid, volatile oil, anthraquinones, and flavonoids. The study suggests that RYNXC was an effective Chinese herbal formula for mammary precancerous lesions and provides a scientific basis for the quality standard and the pharmacology of RYNXC. It will be beneficial to the future clinical application of RYNXC.