Induction of Cell Cycle Arrest and Apoptotic Response of Head and Neck Squamous Carcinoma Cells (Detroit 562) by Caffeic Acid and Caffeic Acid Phenethyl Ester Derivative

Figure 3

Effect of CA and CAPE substances on Detroit 562 cell apoptosis (representative plots). Early apoptotic cells are shown in the lower-right quadrant of the scatter plot, and live cells are in the lower-left quadrant. Both phenolic compounds CA and CAPE induced apoptosis in a dose-dependent manner as measured by the Muse Annexin V and Dead Cell assay. Flow cytometry was shown to induce apoptotic cell death in the epithelial tumor cells Detroit 562 by mainly early and late apoptosis, which was apparent when the percentage of live cells markedly decreased.