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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 6832789, 7 pages
https://doi.org/10.1155/2017/6832789
Research Article

Antigiardial Effect of Kramecyne in Experimental Giardiasis

1Parasitology Research Laboratory, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Col. Doctores, Delegación Cuauhtémoc, 06720 Ciudad de México, Mexico
2Biological Systems Department, UAM Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Delegación Coyoacán, 04960 Ciudad de México, Mexico

Correspondence should be addressed to Enedina Jiménez-Cardoso; xm.moc.oohay@eczenemij

Received 8 August 2017; Revised 11 October 2017; Accepted 16 November 2017; Published 13 December 2017

Academic Editor: Jae Youl Cho

Copyright © 2017 Leticia Eligio-García et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A variety of drugs are used in giardiasis treatment with different levels of efficiency, presence of side effects, and even formation of resistant strains, so that it is important to search new only-one-dose treatments with high efficiency and less side effects. Kramecyne, an anti-inflammatory compound isolated from methanolic extract of Krameria cytisoides, does not present toxicity, even at doses of 5,000 mg/kg. The objective was to determine the antigiardial effect of kramecyne over Giardia intestinalis in vitro and in vivo and analyze the expression of genes ERK1, ERK2, and AK on kramecyne treated trophozoites by Real Time Polymerase Chain Reaction (RTPCR). The median lethal dose (LD50) was 40 μg/mL and no morphological changes were observed by staining with blue trypan and light microscopy; experimental gerbil infection was eliminated with 320 μg/Kg of weight. After treatment there were no differences between intestines from treated and untreated gerbils. Kramecyne did not have significant effect over ERK1 and AK, but there are differences in ERK2 expression (). Results show antigiardial activity of kramecyne; however the mode of action is still unclear and the evaluation of ultrastructural damage and expressed proteins is an alternative of study to understand the action mechanism.