Research Article

Comparative Phytochemical Profiling and In Vitro Antioxidant Activity of Extracts from Raw Materials, Tissue-Cultured Plants, and Callus of Oroxylum indicum (L.) Vent.

Table 1

Total phenolic and total flavonoid contents and in vitro antioxidant activity of extracts from various parts and different growth stages of O. indicum.

SampleYield (% w/w)Total phenolic content
(g GAE/100 g extract)
Total flavonoid content
(g QE/100 g extract)
Antioxidant activity
(EC50, μg/mL)

OIMS17.547.31 ± 0.925.31 ± 0.7373.81 ± 1.89
OIWF26.114.57 ± 0.454.68 ± 0.1265.89 ± 5.48
OIFL116.126.30 ± 0.465.83 ± 0.2848.14 ± 0.92
OIFL215.462.15 ± 0.243.58 ± 0.1771.69 ± 3.01
OIFL339.912.97 ± 0.313.33 ± 0.1084.64 ± 2.49
OIFL430.522.63 ± 0.332.41 ± 0.0426.04 ± 0.59
OIL22.024.10 ± 0.704.59 ± 0.3263.21 ± 4.79
OIPC11.105.66 ± 0.453.24 ± 0.1823.66 ± 1.47
OIS11.632.31 ± 0.223.32 ± 0.1017.69 ± 1.55
OITPd317.802.74 ± 0.591.25 ± 0.08162.38 ± 6.34
OITPd518.537.79 ± 1.022.30 ± 0.2169.61 ± 2.67
OITPd725.347.19 ± 0.852.80 ± 0.1768.96 ± 0.36
OITPw227.836.01 ± 0.292.34 ± 0.1295.03 ± 1.06
OITPw331.735.50 ± 0.460.94 ± 0.24119.42 ± 3.08
OITPw431.471.93 ± 0.540.73 ± 0.08156.97 ± 4.16
OITPw5R10.380.74 ± 0.140.19 ± 0.06500.98 ± 12.25
OITPw5S24.553.82 ± 0.181.30 ± 0.11117.49 ± 19.81
OITPw6R18.901.17 ± 0.05Cannot be detected435.21 ± 2.30
OITPw6S19.172.72 ± 0.351.26 ± 0.11205.60 ± 2.25
OITPw7R16.112.43 ± 0.52Cannot be detected454.36 ± 9.15
OITPw7S18.452.67 ± 0.140.03 ± 0.00251.63 ± 2.95
OITPw8R19.101.44 ± 0.08Cannot be detected607.57 ± 4.52
OITPw8S20.121.72 ± 0.402.32 ± 0.2785.19 ± 2.39
OICB25.126.90 ± 0.452.73 ± 0.0338.78 ± 1.32
OICG26.835.09 ± 1.002.66 ± 0.0798.55 ± 1.92
OICW27.307.05 ± 0.902.61 ± 0.0663.62 ± 3.32
OICY24.646.41 ± 0.912.63 ± 0.0358.64 ± 2.59
Ascorbic acid5.10 ± 0.57
Chrysin>500
Baicalein8.98 ± 0.86
Baicalin32.49 ± 12.52

Different letters in the same column are significantly different ; OIMS = OI seed extract, OIWF = OI whole fruit extract, OIFL1 = OI flower stage 1 extract, OIFL2 = OI flower stage 2 extract, OIFL3 = OI flower stage 3 extract, OIFL4 = OI flower stage 4 extract, OIL = OI leaf extract, OIPC = OI pedicel extract, OIS = OI stalk extract, OITPd3 = OI tissue-cultured plant day 3 extract, OITPd5 = OI tissue-cultured plant day 5 extract, OITPd7 = OI tissue-cultured plant day 7 extract, OITPw2 = OI tissue-cultured plant week 2 extract, OITPw3 = OI tissue-cultured plant week 3 extract, OITPw4 = OI tissue-cultured plant week 4 extract, OITPw5R = OI tissue-cultured plant week 5 root extract, OITPw5S = OI tissue-cultured plant week 5 shoot extract, OITPw6R = OI tissue-cultured plant week 6 root extract, OITPw6S = OI tissue-cultured plant week 6 shoot extract, OITPw7R = OI tissue-cultured plant week 7 root extract, OITPw7S = OI tissue-cultured plant week 7 shoot extract, OITPw8R = OI tissue-cultured plant week 8 root extract, OITPw8S = OI tissue-cultured plant week 8 shoot extract, OICB = OI brown callus extract, OICG = OI green callus extract, OICY = OI yellow callus extract, and OICW = OI white callus extract.