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Evidence-Based Complementary and Alternative Medicine
Volume 2017, Article ID 7074147, 10 pages
Research Article

Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells

1Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China
2National Research Center of Bee Product Processing, Ministry of Agriculture, Beijing 100093, China
3College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Correspondence should be addressed to Liping Sun; moc.621@nusaac

Received 12 December 2016; Accepted 2 February 2017; Published 27 February 2017

Academic Editor: Salvatore Chirumbolo

Copyright © 2017 Liping Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chinese propolis (CP) is known as a health food but its beneficial effects in protecting cardiomyocytes remain elusive. Here, we investigated the effects of CP and its active compounds on hydrogen peroxide (H2O2) induced rats cardiomyocytes (H9c2) oxidative injury. Cell viability decreases induced by H2O2 were mitigated by different CP extracts using various solvents. From these active fractions, six active compounds were separated and identified. Among tested isolated compound, the cytoprotective activities of three caffeates, caffeic acid phenethyl ester (CAPE), benzyl caffeate (BZC), and cinnamyl caffeate (CNC), exerted stronger effects than chrysin, pinobanksin, and 3,4-dimethoxycinnamic acid (DMCA). These three caffeates also increased H9c2 cellular antioxidant potential, decreased intracellular calcium ion () level, and prevented cell apoptosis. Overall, the cardiovascular protective effects of the CP might be attributed to its caffeates constituents (CAPE, BZC, and CNC) and provide evidence for its usage in complementary and alternative medicine.