Evidence-Based Complementary and Alternative Medicine / 2017 / Article / Fig 2

Research Article

Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells

Figure 2

(a) Effects of different alcoholic extracts of CP on H9c2 cardiomyocytes cell viability decreases induced by H2O2 (700 μM for 6 h). H9c2 cardiomyocytes were pretreated with 20 μg/mL alcoholic extracts of propolis, that is, 70% ethanol fraction (70E), 95% ethanol fraction (95E), 40% ethanol upper fraction (40EU), and 40% ethanol lower fraction (40EL). Quercetin (5 μM) served as a positive control. versus oxidative injury group, versus control group, and versus 70E group. (b) Effects of different fractions from CP using different solvents on H9c2 cardiomyocytes cell viability decreases induced by H2O2 (700 μM for 6 h), that is, petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EtOAc), and acetone fractions. Quercetin (5 μM) served as a positive control. versus oxidative injury group, versus control group, and versus EtOAc group. (c) Effects of different subfractions (fractions 1 to 7) from CP EtOAc/acetone fraction on H9c2 cardiomyocytes cell viability decreases induced by H2O2 (700 μM for 6 h). versus oxidative injury group, versus control group, and , versus fraction 3 group.
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