Evidence-Based Complementary and Alternative Medicine / 2017 / Article / Fig 5

Research Article

Potential Protective Effects of Bioactive Constituents from Chinese Propolis against Acute Oxidative Stress Induced by Hydrogen Peroxide in Cardiac H9c2 Cells

Figure 5

Caffeate derivatives in CP decreased the H2O2 induced elevation of and inhibited cell apoptosis in H9c2 cardiomyocytes. (a) Effects of CP bioactive compounds (BZC, CAPE, and CNC) on intracellular induced by H2O2. H9c2 cells were pretreated with BZC, CAPE, and CNC for 12 h and then stimulated with 6 h H2O2 (700 μM). was measured as described in materials and methods. (b–d) Effects of CP bioactive compounds on cell apoptosis induced by H2O2. Various concentrations (1, 5, and 10 μM) of BZC (b), CAPE (c), and CNC (d) were added into cell medium for 12 h and then stimulated with 6 h H2O2 (700 μM). Cell apoptotis were analysed using FCM as described in Materials and Methods. versus oxidative injury group and versus control group.

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