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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 7174858, 6 pages
https://doi.org/10.1155/2017/7174858
Research Article

Anticancer Effects of the Marine Sponge Lipastrotethya sp. Extract on Wild-Type and p53 Knockout HCT116 Cells

1College of Pharmacy, Duksung Women’s University, Seoul 01369, Republic of Korea
2Innovative Drug Center, Duksung Women’s University, Seoul 01369, Republic of Korea

Correspondence should be addressed to Joohee Jung; rk.ca.gnuskud@eehooj

Received 13 October 2016; Revised 8 December 2016; Accepted 21 December 2016; Published 3 January 2017

Academic Editor: Hilal Zaid

Copyright © 2017 Kiheon Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Interest in marine bioresources is increasing in the drug development sector. In particular, marine sponges produce a wide range of unique metabolites that enable them to survive in challenging environments, which makes them attractive sources of candidate pharmaceuticals. In previous study, we investigated over 40 marine specimens collected in Micronesia and provided by the Korean Institute of Ocean Science and Technology, for their antiproliferative effects on various cancer cell lines, and Lipastrotethya sp. extract (LSSE) was found to have a marked antiproliferative effect. In the present study, we investigated the mechanism responsible for its anticancer effect on wild-type p53 (WT) or p53 knockout (KO) HCT116 cells. LSSE inhibited cell viability and induced apoptotic cell death more so in HCT116 p53 KO cells than the WT. HCT116 WT cells treated with LSSE underwent apoptosis associated with the induction of p53 and its target genes. On the other hand, in HCT116 p53 KO cells, LSSE reduced mTOR and Bcl-2 and increased Beclin-1 and LC3-II protein levels, suggesting autophagy induction. These results indicate that the mechanisms responsible for the anticancer effect of LSSE depend on p53 status.