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Figure 4: Effect of Lipastrotethya sp. extract on the expression of cell death-related proteins. Cells treated with Lipastrotethya sp. extract were incubated for 24 h. (a) p53 level and p21 level in HCT116 and HCT116 p53KO cells treated with Lipastrotethya sp. extract. (b) Apoptosis-related protein expression was analyzed by western blotting in HCT116 cells treated with Lipastrotethya sp. extract. (c) Autophagy-related protein expression was analyzed by western blotting in HCT116 and HCT116 p53KO cells treated with Lipastrotethya sp. extract.