Potential Antitumor Activity and Apoptosis Induction of Glossostemon bruguieri Root Extract against Hepatocellular Carcinoma Cells

Figure 5

The effect of MRE treatment on p53, PCNA, p21, Fas, Bax, and PARP expressions. (a) Protein expression levels were assessed by Western blot analysis in HepG2 and Hep3B cells after exposing cells with the indicated concentrations of MRE for 48 h. 50 μg of total cell lysate from each sample was subjected to 10% SDS-PAGE. Proteins were transferred to PVDF and probed with the indicated antibodies. Anti-β-actin was used as a loading control. Protein expressions were quantified, normalized to β-actin and controls. Numbers represent fold of change. (b) Relative protein expression levels in HepG2 and Hep3B cells were quantified using Quantity One software. The experiment was done in triplicate. Data are presented as mean ± SD. P value was calculated versus control cells: and .

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