Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells

Figure 6

Effects of WMW on the expression of proteins related to NLRP3 inflammasome in HepG2 cells. HepG2 cells were treated with serum without WMW (N) or medicated serum containing low (L), middle (M), or high (H) dose of WMW for 24 h. Then, cells were stimulated with 5 μM nigericin for 45 min, and cell extracts were collected and analyzed by western blotting for measurement of NLRP3 (a), ASC (b), caspase-1 (p20) (c), and IL-1β (d) expression. Immunofluorescence staining for caspase-1 (p20) (red) and IL-1β (red) in HepG2 cells from the control group, disease group (PA), and group cotreated with serum containing high dose of WMW (PA + WMW) is shown in ((e) and (f)). The nuclei were counterstained with DAPI (blue). Signals for caspase-1 (P20) and IL-1β are presented in a bar graph ((g) and (h)). Scale, 100 μm. , , and .

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