Regulating the Balance of Th17/Treg via Electroacupuncture and Moxibustion: An Ulcerative Colitis Mice Model Based Study

Figure 5

Flow cytometric pictures and the percentage of CD4⁺CD25⁺Foxp3⁺Treg cells and CD3⁺CD8⁺IL-17⁺Th17 cells among CD4⁺T cells in each group. Lymphocytes from spleen of each group were stained with labeled as anti-mice antibodies as in the described methods. Treg cells and Th17 were gated and shown in the right-upper quadrant. Compared with control group, the percentage of Treg (CD4⁺CD25⁺Foxp3⁺Treg/CD4+ T cells) in model group decreased (). Treg cells content increased after acupuncture or moxibustion treatment (). On the contrary, the percentage of Th17 cells significantly increased () in UC model mice while decreasing by both acupuncture and moxibustion. .