Kyungheechunggan-Tang-01, a New Herbal Medication, Suppresses LPS-Induced Inflammatory Responses through JAK/STAT Signaling Pathway in RAW 264.7 Macrophages

Figure 9

KCT-01 inhibits ear inflammation induced by croton oil in rats. (a) KCT-01 (50 or 100 mg/kg, p.o.) was administered 1 h prior to croton oil treatment and 6 mm ear punch was measured for thickness at 3 h after croton oil application to assess ear edema. ((b) and (c)) Epidermal thickness of ear tissues and histological analysis of croton oil-induced ear edema. Representative H&E sections of ear tissues were obtained from vehicle-treated control rat’s left ear (control), croton oil treated rat’s right ear, and KCT-01 (50 or 100 mg/kg, p.o.) plus croton oil treated rat’s right ear. ((d) and (e)) Ear tissues were homogenized and lysates were used to determine MPO activity, protein expression, and phosphorylation of iNOS, COX-2, and STAT3. Values are expressed as means ± SD. versus control; , , and versus croton oil treated group.

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