Curcumin Suppresses Intestinal Fibrosis by Inhibition of PPARγ-Mediated Epithelial-Mesenchymal Transition

Figure 3

Curcumin reverses the reduction of TGF-β1-induced PPARγ and promotes nuclear translocation of PPARγ. IEC-6 cells were incubated with curcumin at the indicated doses for 24 hours or rosiglitazone for 1 hour followed by treatment with TGF-β1 for 7 days. (a) Western blotting analysis for PPARγ expression in IEC-6 cells. (b) The ratio of grey values of PPARγ/GAPDH bands, , difference versus TGF-β1 group. (c) Immunofluorescence staining for nuclear translocation of PPARγ. Green indicates PPARγ.

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