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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 8215949, 9 pages
Research Article

Tranquilizing and Allaying Excitement Needling Method Affects BDNF and SYP Expression in Hippocampus

1Jilin Provincial Hospital of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130021, China
2Changchun University of Chinese Medicine, Changchun 130117, China
3Institute of Basic Theory of Chinese Medicine, China Academy of Chinese Medical Science, Beijing 100700, China
4Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Correspondence should be addressed to Fuchun Wang; moc.621@024gnawnehcuf

Received 14 February 2017; Accepted 22 May 2017; Published 6 July 2017

Academic Editor: Ki-Wan Oh

Copyright © 2017 Peng Zheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Sleep disorder is a state of sleep loss caused by various reasons, which leads to a series of changes, such as emotion, learning and memory, and immune function. “Tranquilizing and allaying excitement” was widely used in clinical treatment of insomnia; however, the mechanism was still not very clear. We randomly divided rats into three groups: control group, sleep deprivation group, and acupuncture treatment group. We observed BDNF and SYP expression in hippocampus in these three groups. Both protein contents and mRNA contents of BDNF and SYP were measured by western blot, immunohistochemistry, and RT-PCR analysis. The sleep deprivation model was established using modified multiple platform sleep deprivation method (MMPM). Our study explored the BDNF and SYP abnormality in hippocampus caused by sleep deprivation and “tranquilizing and allaying excitement” intervention regulated the abnormal expression of BDNF and SYP caused by sleep deprivation on the short run and the long run. Our study provided a molecular evidence that “tranquilizing and allaying excitement” treatment in rats with sleep disorder affects learning and memory ability.