Anticancer Effects of Sinocrassulosides VI/VII from Silene viscidula on HeLa Cells

Figure 5

Sinocrassulosides VI/VII regulated the expression of cell cycle regulators in HeLa cells. (a) The expression of p16, CDK4, CDK6, cyclin D1, pRb, and E2F1 was detected by Western blot analysis. Tubulin was used as a loading control. (b) The quantitative analysis of p16, cyclin D1, and pRb proteins was illustrated as histograms. Data are mean ± SD of three independent experiments. Asterisks show significant difference compared to the control group (). (c) Cell lysates were subjected to immunoprecipitation using anti-Rb or normal IgG antibodies. The immunoprecipitated products were immunoblotted with anti-E2F1 antibodies.

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