Antioxidant, Anti-Inflammatory, and Analgesic Activities of Agrimonia eupatoria L. Infusion

Table 1

Phenolic characterization of AePF from Agrimonia eupatoria, by HPLC-PDA-ESI/.

Peak	Rt (min)	Tentative identification	max (nm)	(m/z)	MS^2	MS^3
					[m/z (relative abundance, %)]
1	14.94	Procyanidin dimer	279	577	451 (18), 425 (100), 407 (84)	407 (100), 273 (5)
2	16.48	Procyanidin trimer	278	865	739 (36), 713 (34), 695 (100), 577 (49), 543 (15), 425 (20), 407 (21), 405 (14)	677 (39), 543 (69), 525 (100)
3	17.48	Catechin	279	289	245 (100), 205 (41), 203 (16), 179 (7)	227 (34), 213 (11), 203 (100), 175 (17), 174 (11)
4	18.07	Procyanidin dimer	279	577	451 (22), 425 (100), 407 (83), 289 (26)	407 (100), 381 (4), 245 (5)
5	18.25	Procyanidin trimer	279	865	739 (20), 695 (100), 587 (36), 577 (46), 575 (23), 451 (23), 425 (14), 407 (20)	525 (100), 203 (53)
6	18.94	Procyanidin tetramer	279	1153	1057 (51), 983 (51), 965 (68), 863 (39), 813 (84), 713 (41), 695 (100), 617 (92)	—
7	19.78	Procyanidin tetramer	279	1153	917 (99), 863 (88), 813 (86), 489 (100)	—
8	20.01	Procyanidin trimer	280	865	739 (13), 738 (14), 695 (100), 587 (23), 577 (38), 569 (10), 543 (33), 407 (29)	677 (30), 585 (50), 525 (88), 407 (100)
9	21.10	Procyanidin dimer	279	577	451 (12), 425 (100), 407 (75)	—
10	21.65	Procyanidin trimer	280	865	739 (34), 738 (11), 695 (100), 587 (28), 577 (51), 569 (20), 561 (24), 543 (21), 451 (17), 407 (22)	525 (83), 407 (100)
11	23.01	Agrimoniin	258sh, 282	935	899 (3), 633 (100), 523 (2), 463 (5), 301 (93)	301 (100)
12	25.47	p-Coumaric acid	298sh, 310	—	—	—
13	31.01	Apigenin 8-C-glucoside (vitexin)	270, 331	431	341 (3), 311 (100)	293 (3), 283 (100), 239 (4)
14	34.07	Quercetin O-galloyl-hexoside	259sh, 265, 342	615	463 (100), 301 (9), 300 (3)	301 (100), 300 (93), 296 (2), 283 (2), 179 (1)
15	34.85	Apigenin 6-C-glucoside (isovitexin)	270, 333	431	413 (17), 395 (1), 383 (2), 355 (1), 341 (54), 311 (100)	283 (100)
16	35.50	Luteolin 7-O-glucoside (Cynaroside)	268, 295sh, 348	447	327 (6), 285 (100)	285 (100), 257 (85), 242 (37), 241 (42), 217 (60), 198 (62)
17	36.19	Quercetin 3-O-glucoside (isoquercetin)	256, 266sh, 355	463	301 (100), 179 (3)	273 (12), 271 (100), 257 (15), 255 (61), 179 (98)
18	37.35	Quercetin O-malonyl-hexoside	259, 267sh, 348	549	505 (100), 464 (1), 387 (1)	463 (27), 342 (10), 301 (100)
19	38.27	Apigenin 7-O-glucoside (apigetrin)	258sh, 265, 331	431	311 (11), 269 (100), 268 (22)	159 (100)
20	38.77	Ellagic acid	257, 351	301	257 (100), 179 (2), 151 (2)	179 (100), 151 (60)
21	39.42	Kaempferol 3-O-glucoside (astragalin)	265, 346	447	327 (12), 285 (100), 255 (15)	257 (16), 256 (21), 255 (100), 227 (13)
22	40.86	Apigenin O-glucuronide	258sh, 264, 331	445	307 (10), 269 (100), 175 (20)	225 (40), 185 (63)
23	41.53	Kaempferol O-malonyl-hexoside	257sh, 264, 340	533	489 (100)	285 (100)
24	42.31	Kaempferide O-rhamnoside	265, 311, 348	445	298 (100), 283 (5)	269 (100), 207 (22), 191 (7), 187 (4), 163 (24), 148 (6)
25	42.92	Kaempferol O-p-coumaroyl-glucoside (tiliroside)	267, 290sh, 315, 349	593	447 (15), 429 (2), 323 (1), 285 (100), 284 (3), 257 (3)	267 (32), 257 (82), 243 (100), 211 (57)
26	43.56	Kaempferol O-acetyl-hexosyl-O-rhamnoside	267, 294sh, 315, 348	635	575 (21), 489 (20), 431 (11), 349 (5), 285 (100), 255 (3)	257 (100), 255 (42), 240 (8), 213 (9), 183 (9), 151 (21)

sh: shoulder; ; confirmed by commercial reference compounds.