Effects of the Fruit Extract of Tribulus terrestris on Skin Inflammation in Mice with Oxazolone-Induced Atopic Dermatitis through Regulation of Calcium Channels, Orai-1 and TRPV3, and Mast Cell Activation

Figure 6

Effects of TF extract on β-hexosaminidase activity and degranulation in IgE/Ag-stimulated RBL-2H3 cells. The cells were stimulated with anti-DNP-IgE (0.1 μg/mL) for 24 hr, treated with TF extract (0.1, 0.2, and 0.5 mg/mL), DSCG (30 μM/mL), or 2-APB (100 μM/mL) for 1 hr, and then stimulated with DNP-HAS (5 μg/mL) for 3 hr. β-Hexosaminidase activity was measured in culture supernatants using an NAG activity assay kit (a). All data are expressed as the mean ± SD (). and versus normal (A) or DNP-IgE control (B) cells. The cells were stained with toluidine blue and morphological changes were observed under a microscope (×200) (b). The arrows indicate the degranulated cells. N, normal cells; C, DNP-IgE+HSA-stimulated cells; DSCG, DSCG-treated cells; TF0.2, TF extract (0.2 mg/mL)-treated cells; TF0.5, TF extract (0.5 mg/mL)-treated cells; and 2-APB, 2-APB-treated cells.

(a)

(b)