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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 8325049, 12 pages
https://doi.org/10.1155/2017/8325049
Research Article

Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes

1Department of Applied Cosmetology and Master Program of Cosmetic Sciences, Hungkuang University, Taichung, Taiwan
2Department of Nutrition, China Medical University, Taichung, Taiwan
3Department of Medical Laboratory Science and Biotechnology, College of Health Care, China Medical University, Taichung, Taiwan

Correspondence should be addressed to Huey-Chun Huang; wt.ude.umc.liam@gnauhcl

Received 10 December 2016; Revised 21 February 2017; Accepted 6 March 2017; Published 29 March 2017

Academic Editor: Youn C. Kim

Copyright © 2017 Tsong-Min Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Periostracum cicadae is widely used for the treatment of skin diseases such as eczema, pruritus, and itching. The current study sought to evaluate the effect of P. cicadae extract on ultraviolet B (UVB) irradiation and identify the mechanisms involved. Photodamage-protective activity of P. cicadae extracts against oxidative challenge was screened using HaCaT keratinocytes. P. cicadae extracts did not affect cell viability but decreased reactive oxygen species (ROS) production. The extract attenuates the expression of interleukin-6 (IL-6), matrix metalloproteinase-2 (MMP-2), and MMP-9 in UVB-treated HaCaT cells. Also, P. cicadae abrogated UVB-induced activation of NF-κB, p53, and activator protein-1 (AP-1). The downmodulation of IL-6 by P. cicadae was inhibited by the p38 inhibitor (SB203580) or JNK inhibitor (SP600125). Moreover, the extract attenuated the expression of NF-κB and induced thrombomodulin in keratinocytes and thereby effectively downregulated inflammatory responses in the skin. The nuclear accumulation and expression of NF-E2-related factor (Nrf2) were increased by P. cicadae treatment. Furthermore, treatment with P. cicadae remarkably ameliorated the skin’s structural damage induced by irradiation. This study demonstrates that P. cicadae may protect skin cells against oxidative insult by modulating ROS concentration, IL-6, MMPs generation, antioxidant enzymes activity, and cell signaling pathways.