Research Article

Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes

Figure 3

Effects of P. cicadae and herb extracts on UVB-stimulated transcription factors activated in HaCaT cells. (a) The p53 and p65 levels in cell nuclei were determined by Western blotting and lamin B1 was used as a loading control. The protein expression levels of p53 (gray bar) or p65 (white bar) were presented. For gray bar (p53), stands for versus control. For white bar (p65), stands for versus control, stands for versus control, and stands for versus control. The relative expression levels of p53 (gray bar) and p65 (white bar) were compared to control cells and presented as the mean ± SD for three independent experiments performed in triplicate. (b) Relative Nrf-2 mRNA levels were measured by Q-PCRs following UVB and PC (20 μg/mL). Histograms represent mean ± SEM of relative mRNA levels after normalization with 18S (-5 independent experiments). (c) Nrf-2 was revealed by green fluorescence and DAPI was revealed by blue fluorescence. Confocal microscope analysis was used to demonstrate the Nrf-2 accumulation in the nuclear fraction in HaCaT cells. (d) The c-jun and c-fos levels in cell nuclei were determined by Western blotting and lamin B1 was used as a loading control. The relative expression levels were compared to control cells and presented as the mean ± SD for three independent experiments performed in triplicate. Trolox was used as an antioxidant control. H2O2 were used to generate hydroxyl radicals. DSR, Divaricate saposhnikovia root; TT, Tribulus ter; PC, P. cicadae; GGT, Ge Gen Tang. or ,   or , and or were considered as a significant difference compared to control group ( versus control).
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