Antineuroinflammatory Effects of Modified Wu-Zi-Yan-Zong Prescription in β-Amyloid-Stimulated BV2 Microglia via the NF-κB and ERK/p38 MAPK Signaling Pathways

Figure 3

MWP inhibits NF-κB (p65) nuclear translocation in Aβ-activated BV2 microglia. BV2 microglia were incubated with Aβ (20 μM) in the presence or absence of MWP (200 mg/L) for 1 h. Immunofluorescence analysis using an NF-κB (p65) antibody was performed. We used confocal laser scanning microscopy to observe the translocation of NF-κB (p65; red) into the nucleus (blue; scale bar = 25 μm) (a). Western blot showed that MWP inhibited the nuclear translocation of NF-κB (p65) in Aβ-activated BV-2 microglia (b). Data are presented as mean ± standard deviation from independent experiments performed in triplicate. relative to the control group; relative to the Aβ group.

(a)

(b)