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Evidence-Based Complementary and Alternative Medicine
Volume 2017 (2017), Article ID 8671271, 11 pages
https://doi.org/10.1155/2017/8671271
Research Article

PC2 Ovotransferrin: Characterization and Alternative Immunotherapeutic Activity

1S.C. Romvac Company S.A., Soseaua Centurii 7, Voluntari, 77190 Ilfov, Romania
2Department of Pharmacology and Pharmacy, Faculty of Veterinary Medicine, Banat’s University of Agriculture and Veterinary Medicine “King Michael I of Romania” from Timișoara, 119 Calea Aradului, 300645 Timișoara, Romania

Correspondence should be addressed to Romeo Teodor Cristina; moc.oohay@anitsirctr

Received 16 January 2017; Revised 22 February 2017; Accepted 26 February 2017; Published 20 March 2017

Academic Editor: Mariangela Rondanelli

Copyright © 2017 Constantin Chiurciu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Characterization and evaluation of immunotherapeutic potential of ovotransferrin PC2 (OTf PC2) were performed in this study. The ovoprotein was obtained from egg white from hens immunized with bacterial antigens, pathogenic for humans. For the negative control samples, OTf was extracted from eggs collected from Specific Pathogen-Free (SPF) hens and purified by affinity chromatography on Protein G-agarose column with two eluting peaks: I, representing ovalbumin, and II, ovotransferrin. The final apo-OTf form was reached by successive precipitation with ammonium sulfate and citric acid and the holo-OTf form by saturating the apo-form with FeCl3. Multiple OTf PC2 samples were analyzed through Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and, based on the molecular marker migration model, the ovotransferrin (76.5 kDa) and ovalbumin (45 kDa) were detected. The agglutination reaction exhibited statistically significant high specificity of the multiple OTf PC2, by reacting with the antigens used for hens’ immunization. Following ELISA, it was established that OTf PC2 from hyperimmune eggs has specificity for all antigens; the antibody titer was high, indicating that OTf PC2 possesses immunological properties similar to immunoglobulin Y (Ig Y). This study suggests that OTf PC2 immunological activity may play a crucial role in the prevention and treatment of infections resistant to antibiotics and OTf PC2 can also act as a valuable nutraceutical.