Table 5: Structure-activity relationship analysis results.

Hypoglycemic activityStructure-activity relationship
Active groupsInert groups

Genistein > formononetin > biochanin A4′-OH is a strong active group4′-OCH3 and 5-OH were groups that prevented the production of strong activity
1b > Genistein > formononetin > 1a > 1d > biochanin A > 1c4′,7-Diacetyl isoferulic acid in genistein7-Acetyl isoferulic acid in genistein, biochanin A, and formononetin
Genistein > formononetin > 2a > biochanin A > 2b > 2d > 2cnone4′,7-di-L-Aspartic acid dimethyl ester in genistein; 7-L-aspartic acid dimethyl ester in genistein, biochanin A, and formononetin
Genistein > 3c > formononetin > 3d > 3e > 3f > 3g > biochanin A > 3h > 3b > 3a7-Diethyl phosphite in biochanin A7-Diethyl phosphite in genistein, and formononetin; 4′-diethyl phosphite in genistein; 7-diisopropyl phosphite in genistein, biochanin A, and formononetin; 4′-diisopropyl phosphite in genistein
Genistein > 4a > formononetin > 4g > 4f > 4d > 4b > 4j > 4c > 4h > 4i > biochanin A > 4e7-2-Bromo-ethoxy in biochanin A7,4′-Di-2-bromo-ethoxy or 7-2-bromo-ethoxy or 4′-2-bromo-ethoxy in genistein; 7-2-bromo-ethoxy in formononetin;
7,4′-Di-6-bromo-hexyloxy or 7-6-bromo-hexyloxy or 4′-6-bromo-hexyloxy in genistein; 7-6-bromo-hexyloxy in biochanin A; 7-6-bromo-hexyloxy in formononetin
Genistein > formononetin > 5c > 5d > biochanin A > 5b > 5a7-Cinnamic acid in biochanin A7-Cinnamic acid in genistein, and formononetin; 4′-cinnamic acid in genistein
6 > Genistein > formononetin > biochanin AV2+