Research Article

Activation of AMPK by Buddleja officinalis Maxim. Flower Extract Contributes to Protecting Hepatocytes from Oxidative Stress

Figure 8

Effects of BFE and its compounds on AA + iron-induced cell death, AMPK inactivation, and mitochondrial dysfunction. (a) Chemical structures of the BFE compounds. (b) The effects of BFE and its compounds on cell viability and AMPK activation. The cytoprotective effects of BFE (30 μg/mL) and its compounds (10 μM each) against AA + iron with or without compound C (10 μM) were evaluated using the MTT assay. (c) The effects of BFE and its compounds on mitochondrial dysfunction. After staining the cells with 0.05 μg/mL of Rh123 for 30 min, the fluorescence intensity was measured with FACS and RN1 fractions are represented as a percentage of the total. All data represent means ± SD of three independent experiments [(b) between the control and AA + iron-treated cells; and between the AA + iron-treated cells without or with BFE or its compounds with or without compound C; (c) between control and AA + iron-treated cells; and between AA + iron-treated cells without or with BFE and its compounds].
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