Recuperating Lung Decoction Attenuates the Oxidative Stress State of Chronic Obstructive Pulmonary Disease by Inhibiting the MAPK/AP-1 Signal Pathway and Regulating γ-GCS

Figure 3

The phosphorylation levels of ERK, c-jun N-terminal kinase JNK, and p38MAPK are reduced after RLD treatment. (a) The expression levels and locations of p-ERK, p-JNK, and p-p38 in lung tissues were analyzed by immunohistochemistry. Brown positive expression was shown at the bronchial wall and alveolar septum. After RLD treatment, the positive expression of phosphorylation was reduced. (b) A semiquantitative analysis on p-ERK, p-JNK, and p-p38MAPK in lung tissues and an intergroup comparison were conducted. Stimulation of ROS significantly upregulates p38MAPK, ERK, and JNK phosphorylation. The RLD treatment effectively inhibited the phosphorylation levels of p38 MAPK and ERK. RLD showed no obvious influences on phosphorylation level of JNK. The data were expressed as mean ± SD. One-way ANOVA was adopted for statistical analysis. The Newman-Keuls was used for an intergroup comparison (, compared with the normal group, , compared with the model group; ). IOD, integrated optical density.

(a)

(b)