Recuperating Lung Decoction Attenuates the Oxidative Stress State of Chronic Obstructive Pulmonary Disease by Inhibiting the MAPK/AP-1 Signal Pathway and Regulating γ-GCS

Figure 6

RLD reduces the expression of γ-GCS-h in lung tissues. (a-b) γ-GCS-h expression and location were analyzed by immunohistochemistry. Brown positive expression can be seen at the bronchial wall and alveolar septum. After RLD treatment, positive expression of protein was significantly reduced. (c) The γ-GCS-h proteins expressions were analyzed by Western blot. The proteins expressions in COPD group were increased. The expression was reduced after RLD treatment. (d) A quantitative analysis on the γ-GCS-h protein in lung tissues and an intergroup comparison were performed by Western blot. The results were consistent with that from immunohistochemistry. (e) γ-GCS-h mRNA level in tissues was tested by RT-PCR. The level of γ-GCS-h mRNA was higher in COPD group but lower after RLD treatment. The data were shown as mean ± SD. One-way ANOVA was adopted for statistical analysis. The Newman-Keuls was used for an intergroup comparison. (, compared with the normal group, , compared with the model group; ).

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