Research Article

Bioactivity-Guided Fractionation of the Traditional Chinese Medicine Resina Draconis Reveals Loureirin B as a PAI-1 Inhibitor

Figure 2

Effect of loureirin B on PAI-1 activity (LB represents loureirin B in the figures). (a) Inhibition of PAI-1 activity by loureirin B. Data are the means ± SEs from four independent experiments. (b) Inhibition of PAI-1/uPA complex formation by loureirin B. PAI-1 and uPA alone or together and in the presence of the indicated concentrations (0–100 μM) of loureirin B. Protein samples were analyzed by SDS-PAGE under nonreducing conditions and visualized by staining with Coomassie brilliant blue. (c) Promotion of uPA-mediated fibrin clot lysis by loureirin B. (d) Recovery of uPA activity by increasing concentrations of loureirin B. Data are the means ± SEs from five independent experiments. “” indicates significant difference at the level.
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