Aristolochic Acid-Induced Autophagy Promotes Epithelial-to-Myofibroblast Transition in Human Renal Proximal Tubule Epithelial Cells

Figure 2

Temporal dynamic changes of AA-induced autophagy and EMT in HKC cells. HKC cells were incubated with 10 μmol/L AA for different durations. (a) and (b) After stimulation with AA, HKC cells were harvested to detect the protein expression of LC3-II, Beclin 1, E-cadherin, and α-SMA by Western blot assay. The relative protein expression level is expressed as the ratio of target protein/β-actin. Experiments were repeated 5 times independently. Values are represented as mean ± SD (). , compared with control group.

(a)

(b)