Figure 3: E. coli No. 12 biofilm adherence and thickness. Bacteria and L. mutabilis extract were added to LB medium without salt with supplementation of 0 mM and 5 mM of glucose, respectively. Bacteria were incubated with 5% CO2 for 72 h before being washed and stained with crystal violet for spectrophotometry. Data shown are mean +/- SEM. P < 0.0001. Biofilm assays were calculated from at least two independent experiments with six replicates per condition.