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Evidence-Based Complementary and Alternative Medicine
Volume 2018 (2018), Article ID 1364617, 11 pages
Research Article

Antigout Effects of Plantago asiatica: Xanthine Oxidase Inhibitory Activities Assessed by Electrochemical Biosensing Method

1The Research Center of Chinese Medicine Resource and National Medicine of Jiangxi University of Traditional Chinese Medicine, No. 818, Xingwan Road, Nanchang 330004, China
2Institute of Clinical Pharmacology, Hunan Key Laboratory of Harmacogenetics, Central South University, Changsha 410078, China
3School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China
4Beijing University of Chinese Medicine, No. 11, North 3rd Ring Road, Beijing 100029, China
5Tang Center for Herbal Medicine Research and Department of Anesthesia and Critical Care, University of Chicago, 5841 South Maryland Avenue, MC 4028, Chicago, IL 60637, USA

Correspondence should be addressed to Shou-Wen Zhang; moc.361@wsztw

Received 28 July 2017; Revised 9 January 2018; Accepted 16 January 2018; Published 22 February 2018

Academic Editor: Chang G. Son

Copyright © 2018 Jin-Xiang Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The XOD inhibitory effects of Plantaginis Semen, that is, the seeds of P. asiatisca, and its representative four single compounds, acteoside, 1H-indolo-3-carbaldehyde, isoacteoside, and myristic acid, were evaluated by electron transfer signal blocking activities (ETSBA), which is based on the electron transfer signal of XOD enzymatic reaction. The blocking activities were detected using an electrochemical biosensing method. Compared with control, significant effects were observed after the addition of P. asiatica extract, acteoside, and 1H-indolo-3-carbaldehyde (all ). The IC50 values of the extract and acteoside are 89.14 and 7.55 μg·mL−1, respectively. The IC20 values of the extract, acteoside, and 1H-indolo-3-carbaldehyde are 24.28, 3.88, and 16.16 μg·mL−1, respectively. Due to the relatively lower inhibitory potential of 1H-indolo-3-carbaldehyde, its IC50 was not obtained. In addition, isoacteoside and myristic acid did not show any XOD inhibitory effects. Our data demonstrated that the XOD inhibitory effects of the extract, acteoside, and 1H-indolo-3-carbaldehyde can be accurately evaluated by the ETSBA method. The results from this study indicated that Plantaginis Semen significantly inhibited XOD activities to reduce hyperuricemia and treat gout. The study also proves that measuring the electron transfer signal blocking activities is a simple, sensitive, and accurate method to evaluate the XOD inhibitory effects.