Quercetin Potentiates the NGF-Induced Effects in Cultured PC 12 Cells: Identification by HerboChips Showing a Binding with NGF

Figure 5

PC12 cells were serum-starved for 3 hours before treatment of control, 0.5 ng/mL, or 50 ng/mL of NGF and quercetin and cotreatment of quercetin with NGF at 0.5 ng/mL. Cell lysates (20 μg) were subjected to western blotting for phosphorylation analysis. Only 50 ng/mL NGF and the cotreated quercetin with 0.5 ng/mL NGF induced phosphorylation of Erk-1/2 (P-Erk-1/2 at ~42 and ~44 kDa) at 5 min after treatments, no notable changes in phosphorylation were observed in other groups, . The representative gels were shown here.