Research Article
ETAS®50 Attenuates Ultraviolet-B-Induced Interleukin-6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts
Figure 3
Effect of ETAS 50 treatment on UV-B irradiation-induced IL-6 mRNA expression in NHDFs. (a) Time course changes in IL-6 mRNA levels after UV-B irradiation. Cells were cultured for 3–24 h after UV-B irradiation (20 mJ/cm2). The mRNA levels of IL-6 were analyzed by real-time PCR. The expression levels of IL-6 mRNA were calculated as the ratio of its value to that of 18S rRNA, mean ± SEM (n = 3). < 0.05 (by Student’s t-test). (b) Effects of ETAS 50 on UV-B irradiation-induced IL-6 mRNA expression. Cells were treated with 1 mg/mL of ETAS 50 or dextrin (vehicle control) for 24 h after UV-B irradiation (20 mJ/cm2). The mRNA levels of IL-6 were analyzed as described above. (c) Effects of Perifosine on the UV-B irradiation-induced Akt phosphorylation. The cells were treated with 20 μM Perifosine or H2O (vehicle control) for 3 h after UV-B irradiation (20 mJ/cm2). Phosphorylated and total amounts of Akt were detected by western blotting. Phosphorylation levels were calculated as the ratios of the phosphorylated forms to total amount. (d) Effects of Perifosine on the UV-B irradiation-induced IL-6 mRNA expression. The cells were treated with 20 μM Perifosine or H2O (vehicle control) for 24 h after UV-B irradiation (20 mJ/cm2). The mRNA levels of IL-6 were analyzed as described above, mean ± SEM (n = 3). < 0.05 and < 0.01 (by one-way ANOVA and Tukey’s test).
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