Inhibitory Effects of Prunella vulgaris L. Extract on 11-HSD1 in Human Skin Cells

Figure 4

Effects of PVE on activation of GR in cortisone-treated HaCaT cells. (a) The HaCaT cells were cultured in serum-free DMEM, in the presence of an indicated concentration of PVE, for 24 h. NR3C1 mRNA levels were assayed by quantitative real-time PCR. (b) The HaCaT cells were treated with PVE for 1 h, before treatment with cortisone (100 μM). After 40 min of stimulation, the cells were fixed, permeabilized, and incubated with PE-conjugated antihuman GR. GR-PE was analyzed using the IN Cell Analyzer 1000 instrument. The results are mean ± standard deviation (SD) (n = 3). P < 0.001 versus cortisone-untreated control. P < 0.05 versus cortisone-treated control.

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