Bogijetong Decoction and Its Selected Formulation Are Involved in Alleviating Neuropathic Pain in a Rat Model of Chronic Constrictive Injury

Figure 2

Effects of CCI and BGJTD/BeD administration on the production of BDNF and TrkB. (a) Western blot analysis of BDNF in the sciatic nerve. (b, c) Immunofluorescence analysis of BDNF signals in the sciatic nerve. In (b) and (c), longitudinal nerve sections were prepared from CCI+SAL animals. (d) Western blot analysis of TrkB in the sciatic nerve. (e, f) Immunofluorescence staining of TrkB signals in the sciatic nerves. Immunofluorescence images in (e) and (f) were from the longitudinal nerve sections of CCI+SAL animal. In (a) and (d), proximal (P) and distal (D) portions of the nerve were prepared and used for western blotting. Western blotting for actin was used as an internal loading control. Bar graphs (lower panel in (a) and (d)) represent quantification of relative band intensity to actin. Error bar (mean ± SEM, n=4). p<0.05, p<0.01, p<0.001 vs. control group. ⁺⁺⁺p<0.001 vs. corresponding CCI+SAL group. In (c) and (f), areas of suture were marked by asterisks. Scale bar = 100 μm.