Cabbage (Brassica oleracea var. capitata) Protects against H₂O₂-Induced Oxidative Stress by Preventing Mitochondrial Dysfunction in H9c2 Cardiomyoblasts

Figure 1

Cabbage extract protects H9c2 cardiomyocytes against H₂O₂-induced oxidative stress. The MTT assay was performed using (a) cells treated with 10, 50, 100, 200, 300, 500, 1000, and 2000 μg/ml cabbage extract for 48 h or (b) cells pretreated with 100, 200, and 300 μg/ml cabbage extract for 24 h prior to treatment with 500 μM H₂O₂ for another 24 h. (c) Western blot analysis of SOD-1, catalase, and GPx protein expression levels in cells treated with cabbage extract for 48 h. (e) Western blot analysis of Bax, Bcl-2, and cleaved caspase 3 protein expression levels in Cu I-treated cells for 48 h. (d, f) Protein expression levels were quantified by scanning densitometry. β-Actin was used as the loading control. Data are expressed as the % changes ± SEM versus control cells from three independent experiments. Significance was analyzed by a one-way ANOVA followed by the Bonferroni post hoc test. ^###P < 0.001 versus control cells. P < 0.01 and P < 0.001 versus H₂O₂ alone-treated cells. Cont, control; Cab, cabbage extract; NS, not significant.

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