Cabbage (Brassica oleracea var. capitata) Protects against H₂O₂-Induced Oxidative Stress by Preventing Mitochondrial Dysfunction in H9c2 Cardiomyoblasts

Figure 4

Cabbage extract suppresses H₂O₂-induced apoptosis in H9c2 cardiomyocytes. Apoptosis was determined by TUNEL, Hoechst staining, and western blot analysis of apoptosis-related proteins. Representative images of H9c2 cardiomyocytes treated with 500 μM H₂O₂ for 24 h following pretreatment with 100, 200, and 300 μg/ml cabbage extract for 24 h in the (a) TUNEL and (c) Hoechst assays. (b) The apoptotic index was calculated by determining the percentage of (b) TUNEL-positive or (d) Hoechst-positive cells. (e) Western blot analysis of the protein expression levels of Bax, Bcl-2, and cleaved caspase 3. (f) The protein expression levels were quantified by scanning densitometry. β-Actin was used as the loading control. Western blot analysis was performed in triplicate with three independent samples. Data are expressed as fold changes ± SEM versus control cells. Significance was analyzed by a one-way ANOVA followed by the Bonferroni post hoc test. ^### P < 0.001 versus control cells. P < 0.05, P < 0.01, and P < 0.001 versus H₂O₂ alone-treated cells. Cont, control; Cab, cabbage extract. Scale bar, 100 μm.

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