Qi-Dong-Huo-Xue-Yin Inhibits Inflammation in Acute Lung Injury in Mice via Toll-Like Receptor 4/Caveolin-1 Signaling

Figure 3

Effects of QDHXY on the expression levels of inflammatory cytokines and caveolin-1 in the lungs of mice with LPS-induced ALI. Mice were pretreated with QDHXY (0.4 or 0.8 g) or dexamethasone (0.1 mg) daily for 6 days, after which they were treated with 50 μL of 1 mg/mL LPS. The five groups were, respectively, labeled as normal control, ALI, ALI + LDQ (LPS and low-dose QDHXY), ALI + HDQ (LPS and high-dose QDHXY), and ALI + DEX (LPS and dexamethasone). The mRNA levels of IL-6, TNF-α, IL-1β, and caveolin-1 in lung tissue were assayed by real-time PCR using SYBR Green. β-Actin was used as the endogenous control. All the data are ratios against a normal control and have been presented as mean ± SD of three independent experiments. # indicates < 0.01 and ## indicates < 0.05 when compared to the normal control and ALI groups, respectively.

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