Aqueous Extract of Semen Ziziphi Spinosae Exerts Anxiolytic Effects during Nicotine Withdrawal via Improvement of Amygdaloid CRF/CRF1R Signaling

Figure 6

Effects of AESZS on the mRNA expressions of CRF and CRF1R in the CeA during NicW. Immediately after the EPM tests, the mRNA levels of amygdaloid CRF and CRF1R were examined using real-time PCR assays. All data are expressed as a mean ± SEM (n = 5). S: saline; DW: distilled water; Nic: nicotine; NicW: nicotine withdrawal; AESZS: aqueous extract of Semen Ziziphi Spinosae; AESZS180: 180 mg/kg/d AESZS. ^## p < 0.01, ^### p < 0.001, versus S/DW group; p < 0.01, p < 0.001, versus Nic/DW group (one-way ANOVA followed by Newman-Keuls post hoc test).

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